亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Integrating network pharmacology approach and experimental validation to reveal the alleviation of Shenkangning capsule on chronic nephritis

波多辛 药理学 足细胞 局灶节段性肾小球硬化 医学 肾病 肌酐 炎症 肾小球硬化 中医药 肾小球肾炎 蛋白尿 内科学 病理 内分泌学 替代医学 糖尿病
作者
Mengjuan Wei,Zhimiao Qiu,Han Li,Bin Lü,Changhong Wang,Lili Ji
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:299: 115676-115676 被引量:8
标识
DOI:10.1016/j.jep.2022.115676
摘要

Shenkangning (SKN), a Chinese patent medicine composed by eight Chinese medicinal herbs, is commonly applied to treat chronic glomerulonephritis (CGN) in clinic. However, its mechanism is still not clear now.This study is designed to evaluate the SKN-provided alleviation on adriamycin (ADR)-induced nephropathy, to reveal its mechanism by integrating network pharmacology analysis and experimental evidences, and to further find the main drug that makes a major contribution to its efficacy.ADR was intravenously injected to mice to induce focal segmental glomerulosclerosis (FSGS). Renal histological evaluation was conducted. The level of urinary protein, and serum amounts of creatinine, urea nitrogen (BUN) and albumin were detected. The potential mechanisms were predicted by network pharmacology analysis and further validated by Real-time polymerase chain reaction (RT-PCR), Western-blot and enzyme-linked immunosorbent assay (ELISA).SKN (1, 10 g/kg) improved ADR-induced nephropathy in mice. Network pharmacology results predicted that inflammation and oxidative stress were crucially involved in the SKN-provided amelioration on nephropathy. SKN reduced the activation of nuclear factor-κB (NF-κB) and the expression of some pro-inflammatory cytokines, and increased the activation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of its downstream genes in ADR-induced nephropathy in mice. Furthermore, SKN also restored the reduced expression of both podocin and synaptopodin, which are podocyte-associated proteins. Further results showed that the toxic drug Danfupian (DFP) had no contribution to the SKN-provided alleviation on ADR-induced nephropathy in mice. After integrating the results from evaluating anti-inflammation, anti-oxidant and anti-injury of podocytes in vitro and from comparing the activity of the whole SKN and SKN without Astragali Radix (Huangqi, HQ) in vivo, we found that HQ played a crucial contribution to the SKN-provided amelioration on ADR-induced nephropathy in mice.SKN improved ADR-induced nephropathy through suppressing renal inflammation and oxidative stress injury via abrogating NF-κB activation and activating Nrf2 signaling pathway. HQ played a main contribution to the SKN-provided amelioration on ADR-induced nephropathy.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
温暖的豌豆完成签到 ,获得积分10
4秒前
26秒前
陈杰发布了新的文献求助10
26秒前
量子星尘发布了新的文献求助10
26秒前
36秒前
andrele发布了新的文献求助10
36秒前
天天快乐应助jyy采纳,获得200
40秒前
42秒前
科研通AI2S应助妩媚的大门采纳,获得30
46秒前
Uuuu发布了新的文献求助10
48秒前
48秒前
英俊的铭应助taku采纳,获得10
51秒前
57秒前
月半发布了新的文献求助10
57秒前
量子星尘发布了新的文献求助10
58秒前
zhiyuyu完成签到,获得积分10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
1分钟前
月半发布了新的文献求助10
1分钟前
陈杰发布了新的文献求助10
1分钟前
1分钟前
1分钟前
1分钟前
rerorero18发布了新的文献求助10
1分钟前
zhuoak完成签到,获得积分10
1分钟前
nenoaowu应助科研通管家采纳,获得50
1分钟前
量子星尘发布了新的文献求助10
1分钟前
务实书包完成签到,获得积分10
1分钟前
2分钟前
陈杰发布了新的文献求助10
2分钟前
量子星尘发布了新的文献求助10
2分钟前
2分钟前
2分钟前
2分钟前
banbieshenlu发布了新的文献求助30
2分钟前
神勇立果完成签到,获得积分10
2分钟前
2分钟前
wanci应助ting77采纳,获得10
2分钟前
3分钟前
3分钟前
高分求助中
Africanfuturism: African Imaginings of Other Times, Spaces, and Worlds 3000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 2000
Electron microscopy study of magnesium hydride (MgH2) for Hydrogen Storage 1000
Structural Equation Modeling of Multiple Rater Data 700
 Introduction to Comparative Public Administration Administrative Systems and Reforms in Europe, Third Edition 3rd edition 590
全球膝关节骨性关节炎市场研究报告 555
Exhibiting Chinese Art in Asia: Histories, Politics and Practices 540
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3892397
求助须知:如何正确求助?哪些是违规求助? 3435152
关于积分的说明 10791452
捐赠科研通 3160116
什么是DOI,文献DOI怎么找? 1745363
邀请新用户注册赠送积分活动 842857
科研通“疑难数据库(出版商)”最低求助积分说明 786887