Using a Eukaryotic GST Fusion Vector for Proteins Difficult to Express in E. coli

BioTechniquesVol. 23, No. 5 BenchmarksOpen AccessUsing a Eukaryotic GST Fusion Vector for Proteins Difficult to Express in E. coliRobert Y.L. Tsai & Randall R. ReedRobert Y.L. Tsai*Address correspondence to Robert Y.L. Tsai, 725 N. Wolfe St. PCTB 818, Baltimore, MD 21205-2185, USA.Johns Hopkins University School of Medicine, Baltimore, MD, USASearch for more papers by this author & Randall R. ReedJohns Hopkins University School of Medicine, Baltimore, MD, USASearch for more papers by this authorPublished Online:7 Aug 2018https://doi.org/10.2144/97235bm06AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByBiliverdin reductase bridges focal adhesion kinase to Src to modulate synaptic signalingScience Signaling, Vol. 15, No. 733Fat3 acts through independent cytoskeletal effectors to coordinate asymmetric cell behaviors during polarized circuit assemblyCell Reports, Vol. 38, No. 5Disparate Regulatory Mechanisms Control Fat3 and P75NTR Protein Transport through a Conserved Kif5-Interaction Domain27 October 2016 | PLOS ONE, Vol. 11, No. 10Inositol pyrophosphates inhibit synaptotagmin-dependent exocytosis30 June 2016 | Proceedings of the National Academy of Sciences, Vol. 113, No. 29CHD7 interacts with BMP R-SMADs to epigenetically regulate cardiogenesis in mice29 November 2013 | Human Molecular Genetics, Vol. 23, No. 8Characterization of the novel interaction between muskelin and TBX20, a critical cardiogenic transcription factorBiochemical and Biophysical Research Communications, Vol. 409, No. 2Store-Independent Activation of Orai1 by SPCA2 in Mammary TumorsCell, Vol. 143, No. 1S -nitrosylation/activation of COX-2 mediates NMDA neurotoxicity29 July 2008 | Proceedings of the National Academy of Sciences, Vol. 105, No. 30Pirt, a Phosphoinositide-Binding Protein, Functions as a Regulatory Subunit of TRPV1Cell, Vol. 133, No. 3STIM1 carboxyl-terminus activates native SOC, Icrac and TRPC1 channels13 August 2006 | Nature Cell Biology, Vol. 8, No. 9SUMO Modification of Human XRCC4 Regulates Its Localization and Function in DNA Double-Strand Break RepairMolecular and Cellular Biology, Vol. 26, No. 5Transforming Growth Factor β Enhances the Glucocorticoid Response of the Mouse Mammary Tumor Virus Promoter through Smad and GA-Binding ProteinsJournal of Virology, Vol. 78, No. 5Yaf2 inhibits Myc biological functionCancer Letters, Vol. 193, No. 2Protein–protein cross-linking in the use of the eukaryotic eGST-fusion systemProtein Expression and Purification, Vol. 26, No. 3Amino acids 3-13 and amino acids in and flanking the 23 FxxLF 27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor2 December 2002 | European Journal of Biochemistry, Vol. 269, No. 23Identification of mZnf8, a Mouse Krüppel-Like Transcriptional Repressor, as a Novel Nuclear Interaction Partner of Smad1Molecular and Cellular Biology, Vol. 22, No. 21Two Highly Related p66 Proteins Comprise a New Family of Potent Transcriptional Repressors Interacting with MBD2 and MBD3Journal of Biological Chemistry, Vol. 277, No. 43cGMP-dependent Protein Kinase Iβ Physically and Functionally Interacts with the Transcriptional Regulator TFII-IJournal of Biological Chemistry, Vol. 277, No. 35Role of USF1 phosphorylation on cardiac α-myosin heavy chain promoter activityAmerican Journal of Physiology-Heart and Circulatory Physiology, Vol. 283, No. 1Neuronal nitric-oxide synthase localization mediated by a ternary complex with synapsin and CAPON26 February 2002 | Proceedings of the National Academy of Sciences, Vol. 99, No. 5Functional Domains of Histone Deacetylase-3Journal of Biological Chemistry, Vol. 277, No. 11Functional interaction of Yaf2 with the central region of MycN4 October 2001 | Oncogene, Vol. 20, No. 41Identification of Nucleolin as a Glucocorticoid Receptor Interacting ProteinBiochemical and Biophysical Research Communications, Vol. 280, No. 2WbpO, a UDP-N-acetyl-d-galactosamine Dehydrogenase from Pseudomonas aeruginosa Serotype O6Journal of Biological Chemistry, Vol. 275, No. 43Cell Type-specific Regulation of B-Raf Kinase by cAMP and 14-3-3 ProteinsJournal of Biological Chemistry, Vol. 275, No. 41The Androgen Receptor Amino-Terminal Domain Plays a Key Role in p160 Coactivator-Stimulated Gene TranscriptionMolecular and Cellular Biology, Vol. 19, No. 9 Vol. 23, No. 5 Follow us on social media for the latest updates Metrics Downloaded 1,023 times History Published online 7 August 2018 Published in print November 1997 Information© 2018 Author(s)PDF download