Protein‐based Artificial Enzymes

Abstract Originally published in: Artificial Enzymes. Edited by Ronald Breslow. Copyright © 2005 Wiley‐VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3‐527‐31165‐1 The sections in this article are Introduction Artificial Nucleases Based on DNA and RNA Binding Proteins Introduction Artificial Nucleases from Native Protein Scaffolds OP Nuclease Design by Mutagenesis and Chemical Modification Additional Applications for OP Conjugates A Fe‐EDTA Artificial Nuclease Concluding Remarks Catalysts Based on Hollow Lipid‐binding Proteins Lipid‐binding Proteins Initial Work Exploiting the Advantage of a Protein‐based Scaffold Catalytic Turnover with Rate Acceleration Modulation of Cofactor Reactivity with Metal Ions Chemogenetic Approach Adding Functional Groups within the Cavity Scaffold Redesign Hydrolytic Reactions A Flavin‐containing Conjugate Some Limitations Myoglobin as a Starting Point for Oxidase Design Artificial Metalloproteins and Myoglobin Non‐covalent Attachment of a Redox Center Dual Anchoring Strategy Antibodies as Scaffolds for Catalyst Design Antibodies as Specificity Elements Incorporation of an Imidazole Functional Group into an Antibody for Catalysis Comparison of Imidazole‐containing Antibodies Produced by Chemical Modification and Site‐directed Mutagenesis Conclusions