Droplet manipulation holds significant promise across the energy, environmental, and medical fields. However, current methods still lack a solution that simultaneously satisfies the requirements for self-powered energy supply, high efficiency, human-droplet interaction, flexibility, and universality. Herein, we develop a human-droplet interaction platform based on an omni-directional triboelectric tweezer, which directly utilizes triboelectric charges induced by human motion to manipulate droplets. The omni-directional triboelectric tweezer produces the charges and electric field necessary for droplet control through simple sliding motions, thereby eliminating conventional dependencies on power source and complex electrode arrays. Moreover, its omni-directional operation capability further enhances the flexibility and precision of droplet manipulation. Our approach demonstrates effective droplet manipulation in both gas and liquid phases through hand movements, enabling a range of operations such as efficient transportation, precise anchoring, flexible steering, merging chemical reactions, and drug extraction, showcasing its comprehensive application capabilities. Here, authors develop a self-powered omni-directional triboelectric tweezer for precise droplet manipulation. It generates high voltage through hand movements, enabling efficient droplet control in air and oil. Applications may include high-speed transport, precise positioning, and non-invasive sample extraction.