六价铬
铬
铬酸盐转化膜
生物修复
化学
环境修复
核化学
粘质沙雷氏菌
X射线光电子能谱
拉伤
环境化学
细菌
化学工程
生物
生物化学
有机化学
生态学
大肠杆菌
污染
基因
遗传学
解剖
工程类
作者
Amrita Jasu,Bharat Manna,Samir Chandra Das,Buddhadeb Chakraborty,Goutam Pramanik,Rina Rani Ray
标识
DOI:10.1016/j.biortech.2023.130009
摘要
Environmental accumulation of hexavalent chromium [Cr(VI)] in the food chain can induce detrimental effects on plants and animals, which calls for effective remediation strategies using biological entities. The bacterium isolated from an iron mine in Odisha, India, is identified asSerratia marcescensAJRR-22. This multi-metal tolerant strain is capable of bio-converting up to 350 mg/L Cr(VI) within 72 h of incubation. Observable electron dense precipitates in transmission electron microscopic images, data patterns in fluorescence microscopy and flow cytometry clearly reveal the chromate reduction ability of the strain. The molecular study is depicted by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopic analyses. Furthermore, a simulation study to estimate the interactions of chromium bound flavin reductasewith predicted docked complexes suggests significant negative Gibbs free energy and a low inhibition constant (Ki), signifying strong spontaneous binding of Cr(VI) to the enzyme, which makes the strain an efficient candidate for chromium bioremediation.
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