The Impact of Fetal Bovine Serum on the Proliferation of Bone Marrow Derived Mesenchymal Stem Cells

Objective To reveal the impact on the proliferation of bone marrow derived mesenchymal stem cell using fetal bovine serum from different sources.Methods Bone marrow mononuclear cells were separatecl by density gradient centrifugating.Bone marrow derived mesenchymal stem cells from patients with psoriasis and normal controls were cultured with the method of adherent and using two kinds of fetal bovine serum respectively.The times of cells in each group from initial culture were recorded.The proliferation activity of cells from patients with psoriasis was measured by MTT colorimetric assay.Annexin V method was used to examine the spontaneous apoptosis rate of cells from patients with psoriasis.Results Bone marrow derived mesenchymal stem cells from patients with psoriasis emerged gradually apoptosis culturied by fetal bovine serum 1,and were all death finally.Cells from normal controls had a longer time for passage in the same conditions.The cells from patients could be passaged using of fetal bovine serum 2 and the cells from normal controls reduced the time for passaging(compared between the two kinds of serum,P0.05).Conclusion FBS is the important factor that affects the proliferation of bone marrow derived mesenchymal stem cell.