Effects of angelica polysaccharide on the functional status of dendritic cells in HBC trans-genie mice

Objective To investigate the effects of angelica polysaccharide on the functional status of dendritic cells in HBV trans-genie mice. Methods Monocytes of HBV trans-genie mice treated with and without angelic polysaccharide were isolated from spleen and incubated with rmGM-CSF and rmIL-4 to induce dendritic cells generation. The morphotype of dendritic cells was identified by microscope. The phenotype of dendritic cells (CD80,CD86) was characterized by flow cytometry. The capacity of stimulating reaction of allogenic T lymphocytes was detected by MTT assay. The concentration of cytokines such as IL-12 and r-IFN in supernate was tested with ELISA. Results The expression of CD86on the dendritic cells and the concentration of IL-12 and r-IFN in supernate of the dendritic cells of treated group with angelica polysaccharide were all higher than those of control group (P0. 05). The capacity of dendritic cells of treated group to stimulate proliferation of the allogenic lymphocyte was increased in comparison with those of control group (P0. 05). Conclusion Angelica polysaccharide can promote the mature of dendritic cells in HBV trans-genie mice,increasing the expression of CD86 on the dendritic cells and the capacity of stimulating lymphocyte proliferation and excretion of IL-12 and r-IFN. It can strengthen the dendritic cells functions of presenting antigen and stimulate cellular immunological response,and maybe play a role in the anti-virus immunological response of HBV trans-genie mice.