B细胞受体
B细胞
剧目
生物
T细胞受体
幼稚B细胞
抗原
鉴定(生物学)
T细胞
断点群集区域
细胞生物学
抗体
受体
分子生物学
计算生物学
作者
Jacob D. Galson,Dominic F. Kelly,Johannes Trück
标识
DOI:10.1615/critrevimmunol.2016016462
摘要
Advances in next-generation sequencing now allow characterization of the global B-cell receptor (BCR) heavy-chain repertoire at a level that reflects its huge diversity. This technology has provided great insight into the structure of the BCR repertoire and how it responds to specific antigen stimuli. There are numerous potential clinical and research applications of BCR repertoire sequencing, but a major hurdle in the realization of these applications is the identification of the antigen-specific sequences of interest from within the total repertoire. To deconvolute the antigen-specific sequences from total repertoire, either a source of antigen-enriched sequence data is required with which to annotate the total repertoire, or de novo annotation methods must be used based on preconceptions of the features of antigen-specific sequences and their behavior following antigen-specific immune stimulation. We present a review of how these different methods can be applied to identify antigen-specific BCR sequences from the total BCR repertoire.
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