Optimization of Ultrasonic-assisted Extraction Technology of Lentinan Polysaccharides by Response Surface Methodology and its Antioxidant Activity

The response surface methodology was used to optimize the ultrasonic-assisted boiling water extraction of lentinan polysaccharides.Design expert analysis indicated that the optimal processing conditions were:water to solid ratio 37:1,ultrasonic power 550W and ultrasonic extraction time 7.3min,respectively.The extraction yield of lentinan polysaccharide is 20.58% which is close to the predicted value of 22.37%,indicated that using the response surface methodology to optimize the yield of lentinan polysaccharides is reasonable.The optimized extraction technology is better than the traditional hot-water boiling,which increases the yield of lentinan polysaccharides by 33.29% and also decreases the energy consumption by 55.51%.The results showed that the extracting liquid of the lentinus had the capacity to scavenge DPPH·radical and scavenging activity was increased with the polysaccharide concentration.