Application of Homogeneous Design in the Studies of Conditions of Electrotransformation in Saccharomyces cerevisiae

Electroporation is a highly efficient technique introducing extraneous DNA. The method was used to transform Saccharomyces cerevisiae YPH 500. The effects of different factors on the electrotransformation were studied. The results showed that the electric intensity, the concentration of extraneous DNA, and the status of competence significantly affected on transformation efficiency. Homogeneous design experiment was used to analyze by stepwise regression method. The results showed that the transformation ratio of Saccharomyces cerevisiae YPH 500 was 24 ×102transformations/μg DNA under the electric intensity of 1 750 V, OD600 nmof 1.3 and the concentration of extraneous DNA of 7.5 mg/L.