生物
二氢叶酸还原酶
质粒
基因
大肠杆菌
打开阅读框
遗传学
转座因子
分子生物学
肽序列
突变体
作者
Lars Sundström,Catarina Jansson,Kåre Bremer,Elli Heikkilä,Barbro Olsson‐Liljequist,Ola Sköld
出处
期刊:Gene
[Elsevier]
日期:1995-02-01
卷期号:154 (1): 7-14
被引量:37
标识
DOI:10.1016/0378-1119(94)00905-8
摘要
A new plasmid-borne gene, dhfrVIII, encoding high-level trimethoprim resistance (TpR) was found in an intestinal Escherichia coli. It seems to be a widely occurring mediator of TpR. Among 973 examined TpRE. coli, the new resistance gene was found in 13 (1.3%) isolates from Sweden, Finland and Nigeria. The new gene was sequenced and found to code for a dihydrofolate reductase (DHFR) of 169 amino acids (Mr 19005). The dhfrVIII gene on the studied plasmid pLMO226 was observed to be flanked by IS26 elements. The dhfrVIII gene and a 3′ unidentified open reading frame (ORF) seem to be borne on a compound transposon with IS26 at its ends, since the configuration of two IS26 flanking dhfrVIII and the unidentified ORF was conserved among the isolates that were probe-positive for the gene. Phylogeny parsimony analysis showed the dhfrVIII-encoded enzyme to be only remotely related to other known plasmid-mediated, drug-resistant DHFR. Only a few of the latter form well-supported monophyletic groups.
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