核小体
生物
染色质
塔塔盒子
酿酒酵母
抄写(语言学)
染色质免疫沉淀
核苷酸切除修复
高流动性组
遗传学
细胞生物学
分子生物学
DNA修复
DNA
发起人
基因
基因表达
语言学
哲学
作者
Neville G Powell,José Antonio Ferreiro,N. Karabetsou,Jane Mellor,Raymond Waters
出处
期刊:DNA Repair
[Elsevier]
日期:2003-04-02
卷期号:2 (4): 375-386
被引量:23
标识
DOI:10.1016/s1568-7864(02)00239-2
摘要
We have assessed how transcription, chromatin structure and protein binding modulate nucleotide excision repair in the upstream regulatory region and early coding region of the endogenous Saccharomyces cerevisiae gene MET17. Removal of UV-induced cyclobutane pyrimidine dimers was measured from these regions, in which transcription and chromatin structure could be regulated independently of each other. Distinct repair trends were apparent depending on transcriptional state. When transcription was repressed nucleosome positioning and protein binding as determined by chromatin immunoprecipitation and quantitative real-time PCR, were significant factors. Nucleosome positioning and/or protein binding effects were most apparent on the strand that becomes transcribed, with repair occurring fastest in a nucleosome free region but being retarded where regulatory proteins bound within this region. When transcription was derepressed the rate of repair increased on both strands in a region beginning 200 bp upstream of the TATA box and extending downstream into the coding region. This effect overrode the influences of nucleosome positioning and protein binding.
科研通智能强力驱动
Strongly Powered by AbleSci AI