生物正交化学
预定位
标签
体内
西妥昔单抗
化学
单克隆抗体
组合化学
生物化学
抗体
点击化学
生物
生物技术
免疫学
放射免疫疗法
作者
Helen L. Evans,Quang Dé Nguyen,Laurence Carroll,Maciej Kaliszczak,Frazer Twyman,Alan C. Spivey,Eric O. Aboagye
摘要
Herein, we describe a fast and robust method for achieving (68)Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels-Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the method within a two-step pretargeting strategy.
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