尿素酶
戊二醛
生物传感器
化学
固定化酶
离子强度
牛血清白蛋白
尿素
粘附
缓冲器(光纤)
色谱法
纳米技术
酶
化学工程
材料科学
生物化学
有机化学
工程类
水溶液
电信
计算机科学
作者
Bubriak Oa,Khustochka Ln,Soldatkin Ap,Starodub Nf
出处
期刊:PubMed
日期:1992-01-01
卷期号:64 (1): 66-71
摘要
Many-sided investigations of urease immobilization methods were carried out to create the biosensor devices on the base of semiconductor structures. Special attention was concentrated on the biomembrane formation by means of urease and bovine serum albumin (BSA) cross-linking by gaseous glutaraldehyde. Optimal conditions for the formation process were selected which preserve about 20% of total urease activity after the cross-linking. The properties of enzyme immobilized by the above-mentioned method have been comprehensively studied. They included the urease activity dependence on pH, ionic strength, incubation buffer capacity as well as the enzyme stability during its functioning, storing and thermoinactivation. As was shown, for immobilized ureas Km value for urea at pH 7.0 and 20 degrees C is 1.65 time less than for free enzyme. In the presence of EDTA (1 mM) the enzyme activity in the biomembrane is practically unchanged under a month storing. Biomembrane possesses good adhesion to silicon surface and its swelling level under different conditions does not exceed 35%. The conclusion is made about the prospects of the used method of biomembrane formation for biosensor technology based on semiconductor structures.
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