Induction and Ploidy Identification of the Polyploid of Anoectochilus fornosanus

[Objective] To induce the polyploid of Anoectochilus fornosanus and identify its ploidy.[Method] The adventitious buds of Anoectochilus fornosanus were respectively soaked and coated with colchicine to induce the polyploid,and the ploidy of the chromosome was observed under microscope.[Result] The ploidy was 35% when the adventitious buds were coated with 0.05% colchicine for 72 h;the ploidy identification showed that there were 40 diploid chromosomes and 80 tetraploid chromosomes.[Conclusion] The method can cultivate the polyploid of Anoectochilus fornosanusand,improve its survival rate and per mu yield of its transplants.