[Cloning and sequence analysis of the molecular-adjuvant gene of the complement C3d of mouse].

Considering that the complement C3d plays a stronger positive role in the control of immunologic system, we constructed the recombinant plasmid of the molecular-adjuvant gene of the complement C3d in order to enhance the immunologic efficacy of the gene vaccine.The main points were amplifying the segment of complement C3d gene by using of PCR; inserting it into PGEM-T; detecting it by digesting with Eco RI and sequencing; the whole length being 897 bp; then sub-cloning it into pVAX1.The DNA sequencing confirmed the C3d sequence.We have successfully cloned the gene of C3d and constructed the recombinant plasmid of pVAX1 C3d.