Cytosinpeptidemycin was a newly reported antiphytoviral agricultural antibiotics.The control effect of Cytosinpeptidemycin on infectivity of TMV in different plants was tested.The results showed that Cytosinpeptidemycin had a better effect on the infectivity of TMV in all tested plants.The control effect on tobacco virus disease aused by TMV was remarkable and the inhibition rate was 80.4%.Cytosinpeptidemycin can inhibit infectivity of TMV in tomato and hot pepper.