Extraction of Egg DNA in Feces and Establishement of PCR for Detecting Clonorchis sinensis

Clonorchiasis,known as liver fluke disease isa zoonosis caused by Clonorchis sinensis.The parasites can infect various kinds of animals.At present,the isolation and identification of Clonorchis sinensis mainly focused in adults or metacercariae,but little in eggs.The method to extract DNAs in feces infected with Clonorchis sinensis and detect gene products of Clonorchis sinensis by PCR were successfully established,and the PCR system were optimized,which provided scientific basis on the molecular level for detecting eggs of Clonorchis sinensis.