Influence of negative pressure wound therapy on granulation tissue formation and interleukin-6 variation in chronic wound

Objective To investigate the influence of negative pressure wound therapy(NPWT) on vascular endothelial cells,proliferating cells and interleukin-6 variation in chronic wound.Methods Forty patients with chronic wound were divided into negative pressure wound therapy treatment group and conventional treatment group randomly.The granulation tissue formation was observed,and specimens were obtained from wound bed at 1,4,7,14 days after treatment.The specimens were pathologically studied with hematoxylin-eosin staining to assess the wound healing process of the two groups.Furthermore,immunohistochemistry was done to estimate the number of vascular endothelial cells(factor Ⅷ related antigen being used as marker) and proliferating cells(mouse anti-Ki-67 nuclear antigen being used as marker).Exudates of chronic wound were collected at 1,4,7,14 days after negative pressure wound therapy treatment,interleukin-6 contents were determined and comparisons were made between them based on enzyme linked Immunosorbnent assay.Results The wounds of patients in negative pressure wound therapy treatment group were cured rapidly.The granulation tissue formation was promoted in negative pressure wound therapy treatment group.The number of vascular endothelial cells and proliferating cells in negative pressure wound therapy treatment group was larger than that of conventional treatment group(P0.05).Interleukin-6 contents were decreased with time going after negative pressure wound therapy with significant variations between 1,4,7 days(P0.05),and levels of interleukin-6 were got close to normal at 7,14 days.Conclusion Compared with conventional treatment,negative pressure wound therapy could initiate granulation tissue formation more promptly,accelerate endothelial cell cytopoiesis,stimulate cell proliferation and decrease interleukin-6 contents.