鲍曼不动杆菌
噬菌体
微生物学
生物
抗生素耐药性
突变体
抗菌剂
基因
抗生素
体内
噬菌体疗法
病毒学
细菌
大肠杆菌
铜绿假单胞菌
遗传学
作者
Fernando Gordillo Altamirano,John Harper Forsyth,Ruzeen Patwa,Xenia Kostoulias,Michael Trim,Dinesh Subedi,Stuart K. Archer,Faye C. Morris,Cody Oliveira,Luisa Kielty,Denis V. Korneev,Moira K. O’Bryan,Trevor Lithgow,Anton Y. Peleg,Jeremy J. Barr
出处
期刊:Nature microbiology
日期:2021-01-11
卷期号:6 (2): 157-161
被引量:141
标识
DOI:10.1038/s41564-020-00830-7
摘要
We characterized two bacteriophages, ΦFG02 and ΦCO01, against clinical isolates of Acinetobacter baumannii and established that the bacterial capsule is the receptor for these phages. Phage-resistant mutants harboured loss-of-function mutations in genes responsible for capsule biosynthesis, resulting in capsule loss and disruption of phage adsorption. The phage-resistant strains were resensitized to human complement, beta-lactam antibiotics and alternative phages and exhibited diminished fitness in vivo. Using a mouse model of A. baumannii infection, we showed that phage therapy was effective. The authors characterize two phages against Acinetobacter baumannii, both in vitro and in vivo. A. baumannii strains develop resistance against both phages, but are in turn resensitized to different antimicrobial compounds.
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