生物正交化学
自愈水凝胶
去细胞化
生物分子
纳米技术
生物材料
组织工程
细胞外基质
材料科学
微图形化
脚手架
化学
蛋白质工程
三维细胞培养
生物物理学
细胞
点击化学
生物医学工程
组合化学
生物化学
生物
医学
酶
高分子化学
作者
Ivan Batalov,Kelly R. Stevens,Cole A. DeForest
标识
DOI:10.1073/pnas.2014194118
摘要
Hydrogel biomaterials derived from natural biopolymers (e.g., fibrin, collagen, decellularized extracellular matrix) are regularly utilized in three-dimensional (3D) cell culture and tissue engineering. In contrast to those based on synthetic polymers, natural materials permit enhanced cytocompatibility, matrix remodeling, and biological integration. Despite these advantages, natural protein-based gels have lagged behind synthetic alternatives in their tunability; methods to selectively modulate the biochemical properties of these networks in a user-defined and heterogeneous fashion that can drive encapsulated cell function have not yet been established. Here, we report a generalizable strategy utilizing a photomediated oxime ligation to covalently decorate naturally derived hydrogels with bioactive proteins including growth factors. This bioorthogonal photofunctionalization is readily amenable to mask-based and laser-scanning lithographic patterning, enabling full four-dimensional (4D) control over protein immobilization within virtually any natural protein-based biomaterial. Such versatility affords exciting opportunities to probe and direct advanced cell fates inaccessible using purely synthetic approaches in response to anisotropic environmental signaling.
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