[Metabolic Characteristics of WBC-deplted RBC suspension during Different Storage Stage in MAP Based on UPLC-MS/MS].

To explore the metabolic characteristics and metabolic markers of WBC-depleted RBCs in MAP preservation solution and to analyzed the metabolic profile of RBC in MAP preservation solution by using metabolomics.The changes of metabolitcs in 10 U WBC-depleted RBC suspension at 3-different storage period (D 0, D 14 and D 35) were detected by using the UPLC-MS/MS, the charaeteristic ions and metabolic markers of RBC stored in preservation sblution for 0 d, 14 d and 35 days were analyzed by using the principal component analysis(PCA).The number of characteristic ions in RBC and supernatant extracts detected during the initial, middle and final storage could be clearly distinguiseed. The 5 metabolism-related substamces such as lact-c acid, nicotinamide, glucose, 5-htdroxyproline and malic acid showed statistically significant difference in 3 storage period.The UPLC-MS/MS method combined with statistical analysis of multivariate data can be used to study the metabolic characteristics of RBC, the different metabolites of RBC in different storage stages can be used as the potential markers for evaluation of guality of RBC in storag period. The results of this study provide a basis for studing the RBC guality changes in storage period.基于UPLC-MS/MS的去白细胞的悬浮红细胞不同储存阶段代谢特征研究.采用代谢组学的研究方法，分析红细胞在MAP保存液中不同储存阶段的代谢特征，探索红细胞在MAP保存液中的代谢标志物.运用超高效液相色谱-四级杆飞行时间串联质谱（UPLC-MS/MS）技术检测10袋MAP去白细胞的悬浮红细胞在制备当天（d 0）、储存第14 d（d 14）、储存期末（d 35）等3个不同储存阶段红细胞内和上清液中的代谢物变化情况，采用主成分分析法（PCA）分析不同储存时间的红细胞差异性特征离子和代谢标志物.红细胞内和上清液中提取物在存储初期、中期和末期3个储存阶段检测到的特征离子数能够得到明显区分。乳酸、烟碱胺、葡萄糖、5-羟脯氨酸和苹果酸等5种红细胞代谢相关物质在3个储存阶段水平差异均有统计学意义，可以作为红细胞储存期内的代谢标志物.UPLC-MS/MS方法结合多变量数据统计分析可用于红细胞代谢特征研究，红细胞在不同储存阶段的差异性代谢物质可作为红细胞储存期质量检测标志物，本研究结果为研究红细胞储存期质量变化提供依据.