Isolasi dan Identifikasi Gen 16S rRNA Streptomyces Sp. Isolat Tanah Gunung Semeru

Streptomyces sp has been isolated from the antibiotic-producing slopes of Mount Semeru. Soil takenfrom an altitude of 2000 meters to 3500 meters above sea level which is impassable lava flow from Mount Semeru. Then isolation of Streptomyces sp. Special media for growth of Streptomyces sp. is an ISP-4. Based on the research method that has been done, the results obtained from this study are: the optimization of PCR only produce a tape that shows a length of about 1350 bp. It has been found five species of Streptomyces sp isolates. of land around Ranu Pane named Streptomyces sm-2, Streptomyces sm-5 and Streptomyces sm-6, while those found on the ground around Ranu Regulo named Streptomyces sm-7 and Streptomyces sm-9. The conclusion from this study is that there are different types of colonies found in Ranu Pane and Ramt Regulo on the slopes of Mount Semeru and have found 5 strain of Streptomyces sp., Which later was named Streptomyces s sm-2, Streptomyces sm-5, Streptomyces sm-6, Streptomyces 7 and Streptomyces sm-sm-9 Key words: Isolation and Identification, the 16S rRNA gene of Streptomyces sp. Isolate the land of Mount Semeru,