间充质干细胞
干细胞
细胞生物学
神经营养因子
生物
胶质细胞源性神经生长因子
脂肪组织
干细胞移植修复关节软骨
牙髓干细胞
再生医学
成体干细胞
神经球
间充质干细胞的临床应用
免疫学
细胞分化
内分泌学
受体
基因
生物化学
作者
Saeideh Karimi‐Haghighi,Sara Chavoshinezhad,Anahid Safari,Iman Razeghian‐Jahromi,Iman Jamhiri,Zahra Khodabandeh,Sahar Khajeh,Shahrokh Zare,Afshin Borhani‐Haghighi,Mehdi Dianatpour,Sareh Pandamooz,Mohammad Saied Salehi
标识
DOI:10.1016/j.neulet.2022.136511
摘要
During the last 20 years, stem cell therapy has been considered as an effective approach for regenerative medicine. Due to poor ability of stem cells to survive following transplantation, it has been proposed that beneficial effects of stem cells mainly depend on paracrine function. Therefore, the present study was designed to reinforce mesenchymal stem cells (MSCs) to express higher levels of trophic factors especially the ones with the neurotrophic properties. Here, bone marrow (BM)-MSCs and adipose-MSCs were treated with conditioned medium (CM) of dental pulp stem cells (DPSCs) or hair follicle stem cells (HFSCs) for up to three days. The relative expression of five key trophic factors that have critical effects on the central nervous system regeneration were evaluated using qRT-PCR technique. Furthermore, to assess the impacts of conditioned mediums on the fate of MSCs, expression of seven neuronal/glial markers were evaluated 3 days after the treatments. The obtained data revealed priming of BM-MSCs with HFSC-CM or DPSC-CM increases the BDNF expression over time. Such effect was also observed in adipose-MSCs following DPSC-CM treatment. Secretome preconditioning remarkably increased NGF expression in the adipose-MSCs. In addition, although priming of adipose-MSCs with HFSC-CM increased GDNF expression one day after the treatment, DPSC-CM enhanced GDNF mRNA in BM-MSCs at a later time point. It seemed priming of BM-MSCs with HFSC-CM, promoted differentiation into the glial lineage. Our findings showed that MSCs preconditioning with secretome of neural crest-derived stem cells could be a promising approach to enhance the neurotrophic potential of these stem cells.
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