核酸酶
劈开
生物传感器
检出限
微流控
脱氧核糖核酸酶ⅰ
唾液
纳米技术
DNA
计算机科学
化学
计算生物学
色谱法
生物化学
材料科学
生物
基序列
作者
Skomantas Serapinas,Justina Gineitytė,Marius Butkevičius,Rapolas Danilevičius,Marius Dagys,Dalius Ratautas
标识
DOI:10.1016/j.bios.2022.114475
摘要
DNases are enzymes that cleave phosphodiesteric bonds of deoxyribonucleic acid molecules and are found everywhere in nature, especially in bodily fluids, i.e., saliva, blood, or sweat. Rapid and sensitive detection of DNase activity is highly important for quality control in the pharmaceutical and biotechnology industries. For clinical diagnostics, recent reports indicate that increased DNase activity could be related to various diseases, such as cancers. In this paper, we report a new bioelectronic device for the determination of nuclease activity in various fluids. The system consists of a sensor electrode, a custom design DNA target to maximize the DNase cleavage rate, a signal analysis algorithm, and supporting electronics. The developed sensor enables the determination of DNase activity in the range of 3.4 × 10-4 - 3.0 × 10-2 U mL-1 with a limit of detection of up to 3.4 × 10-4 U mL-1. The sensor was tested by measuring nuclease activity in real human saliva samples and found to demonstrate high accuracy and reproducibility compared to the industry standard DNaseAlert™️. Finally, the entire detection system was implemented as a prototype device system utilizing single-use electrodes, custom-made cells, and electronics. The developed technology can improve nuclease quality control processes in the pharmaceutical/biotechnology industry and provide new insights into the importance of nucleases for medical applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI