Multimode determination of uric acid based on porphyrinic MOFs thin films by electrochemical and photoelectrochemical methods

Uric acid (UA) is a significant biological substance for the disorders associated with alterations of the plasma urate concentration such as ketoacidosis, hyperuricemia, renal impairment, leukemia, Lesch-Nyhan syndrome and lactate excess, chronic nephritis, arthrolithiasis, etc. The development of UA ultra-sensitive determination technologies is an essential requirement due to its biological properties. Numerous determination technologies have been explored to analyze UA in various real samples. However, a rapid, easy-to-use, cost-effective and instrument-free biosensor for UA determination remains a great challenge. A facile electrochemical and photoelectrochemical (PEC) sensor was fabricated through metal organic frameworks (MOFs) which were synthesized based on Cu(NO3)2·3H2O and 5,10,15,20-tetrakis (4-carboxyphenyl) porphyrin (H2TCPP) by the environmentally-friendly solvothermal method. Cu-TCPP MOFs/GCE sensing platforms were applied in quantitative determination of UA through the different electrochemical technologies. The electrochemical signal responses of UA decreased regularly with the increase of UA concentration and the well-linear regression equations were established between the peak current intensity and UA concentrations in various electrochemical technologies.