Per- and Polyfluoroalkyl Substances in Semen Associated with Repeated Measures of Semen Quality in Healthy Adult Men

= 1019) from multiple collections (or in a single semen sample if no multiple collection was available). The results reveal seminal perfluorooctanoic acid, perfluorooctanesulfonic acid, perfluorohexanesulfonic acid, and 6:2 chlorinated polyfluoroalkyl ether sulfonate were significantly associated with decreased sperm progressive motility and total motility, while multiple seminal PFAS were positively associated with sperm concentration and total sperm count. By contrast, null associations were observed between plasma PFAS and semen quality. Our study provides epidemiological evidence that PFAS exposure may affect male fertility and seminal PFAS should be measured for precise assessment of the impact of PFAS on male reproductive performances.