Probiotic model for studying rhizosphere interactions of root exudates and the functional microbiome

Abstract Root exudates are important mediators of plant–microbiome interactions. Recent pioneering studies on various aerial root plants, including cereals, have shown that carbohydrate-rich mucilage can enrich diazotrophs and increase host nitrogen utilization and growth. Moreover, non-diazotrophic “gatekeeper” microorganisms in mucilage help defend against pathogenic and environmental microbes. These findings highlight the active role of root exudates in mediating plant–microbiome interactions to maintain microbial homeostasis in the rhizosphere. However, little is known about the specific mechanisms by which root exudates modulate the functional microbiome and homeostasis in rhizosphere microhabitats. Here, we propose the typical and stable biointeractions of four plant–specific aerial root mucilage–probiotic systems as a model for understanding root exudate–functional microbiome interaction. We anticipate that this model can provide fundamental biological insights into rhizosphere interactions.