环境友好型
生物燃料
稻草
稻草
制浆造纸工业
生产(经济)
过氧化物酶
化学
生物能源
环境科学
生化工程
农学
废物管理
有机化学
工程类
生物
酶
生态学
宏观经济学
经济
作者
Kenneth B. K. Teo,Kengo Kondo,Sadat Mohamed Rezk Khattab,Takashi Watanabe,Takashi Nagata,Masato Katahira
标识
DOI:10.1021/acs.jafc.3c07998
摘要
Rice straw (RS), an agricultural residue rich in carbohydrates, has substantial potential for bioethanol production. However, the presence of lignin impedes access to these carbohydrates, hindering efficient carbohydrate-to-bioethanol conversion. Here, we expressed versatile peroxidase (VP), a lignin-degrading enzyme, in Pichia pastoris and used it to delignify RS at 30 °C using a membrane bioreactor that continuously discarded the degraded lignin. Klason lignin analysis revealed that VP-treatment led to 35% delignification of RS. We then investigated the delignified RS by SEC, FTIR, and SEM. The results revealed the changes of RS caused by VP-mediated delignification. Additionally, we compared the saccharification and fermentation yields between RSs treated with and without VP, VP-RS, and Ctrl-RS, respectively. This examination unveiled an improvement in glucose and bioethanol production, VP-RS exhibiting up to 1.5-fold and 1.4-fold production, respectively. These findings underscore the potential of VP for delignifying RS and enhancing bioethanol production through an eco-friendly approach.
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