High-resolution portable lens-free on-chip microscopy with RGB LED via pinhole array

Lens-free on-chip microscopy with RGB LEDs (LFOCM-RGB) provides a portable, cost-effective, and high-throughput imaging tool for resource-limited environments. However, the weak coherence of LEDs limits the high-resolution imaging, and the luminous surfaces of the LED chips on the RGB LED do not overlap, making the coherence-enhanced executions tend to undermine the portable and cost-effective implementation. Here, we propose a specially designed pinhole array to enhance coherence in a portable and cost-effective implementation. It modulates the three-color beams from the RGB LED separately so that the three-color beams effectively overlap on the sample plane while reducing the effective light-emitting area for better spatial coherence. The separate modulation of the spatial coherence allows the temporal coherence to be modulated separately by single spectral filters rather than by expensive triple spectral filters. Based on the pinhole array, the LFOCM-RGB simply and effectively realizes the high-resolution imaging in a portable and cost-effective implementation, offering much flexibility for various applications in resource-limited environments.