棉蚜
益达胺
生物
啶虫脒
有害生物分析
苏力菌棉花
园艺
RNA干扰
棉属
若虫
人口
转基因
转基因作物
植物
锦葵科
毒理
杀虫剂
生物技术
农学
蚜虫科
基因
核糖核酸
同翅目
遗传学
人口学
社会学
作者
Lianjun Zhang,Yuanjie Wei,Wei Linyu,Xiaoning Liu,Ning Liu
摘要
The pest Aphis gossypii Glover globally causes considerable economic losses on various crops by its feeding damage and disease transmission. Transgenic plants that produce double-stranded RNA (dsRNA) targeted to insect genes are being developed as a pest control strategy. In this study, we evaluated the effects of transgenic cotton-mediated RNA interference (RNAi) on the growth and detoxification ability of A. gossypii after the transgenic cotton lines expressing dsAgCYP6CY3-P1 (the TG cotton lines) were obtained on the basis of exploring the functions of CYP6CY3 in our previous research.The developmental time of third- and fourth-instar nymphs which fed on the TG cotton lines were significantly prolonged. Life table parameters showed that the fitness of cotton aphids from the TG cotton lines decreased. Additionally, the relative expression level of CYP6CY3 in cotton aphids which fed on the TG cotton lines was significantly reduced by 47.3 % at 48 h compared with that from the nontransgenic cotton (the NT cotton). Bioassay showed that silencing of CYP6CY3 increased mortality of the nymphs to imidacloprid by 28.49 % (at 24 h) and to acetamiprid by 73.77 % (at 48 h), respectively.These results indicated that the TG cotton lines delayed the growth and development of A. gossypii, but also decreased population density and increased its sensitivity to imidacloprid and acetamiprid, respectively. The results provide further support for the development and application of plant-mediated RNAi. © 2022 Society of Chemical Industry.
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