The experiment was carried out in triplicate in 500 L tanks with three biofloc treatments and one control.Biofloc tanks were filled with 360 L of water and fed sugarcane molasses, wheat flour, Rice flour, and clean water as a control without biofloc for 28 days.The postlarvae of Litopenaeus vannamei (Boone, 1921) were stocked at a rate of 130 PL m -2 and cultured for a period of 50 days while being fed pelleted feed at a rate of 1.5% of biomass.The average body weight of the postlarvae was 0.15 ± 0.02 g.In the biofloc treatments, the addition of carbohydrate greatly boosted the population of total heterotrophic bacteria (THB) while dramatically reducing the total ammonia -N (TAN), nitrite -N, and nitrate -N in the water.The wheat flour treatment had a significantly higher final average body weight (8.17 ± 0.26 g) than the treatment and control groups of shrimp.The treatment had no effect on the shrimp survival rate, which varied from 72.6% to 86.3%.Conclusion: The use of wheat flour effectively increased the formation of biofloc and contributed to better water quality, resulting in higher shrimp production.