Balancing Doses of EL222 and Light Improves Optogenetic Induction of Protein Production in Komagataella phaffii

ABSTRACT Komagataella phaffii , also known as Pichia pastoris , is a powerful host for recombinant protein production, in part due to its exceptionally strong and tightly controlled P AOX1 promoter. Most K. phaffii bioprocesses for recombinant protein production rely on P AOX1 to achieve dynamic control in two‐phase processes. Cells are first grown under conditions that repress P AOX1 (growth phase), followed by methanol‐induced recombinant protein expression (production phase). In this study, we propose a methanol‐free approach for dynamic metabolic control in K. phaffii using optogenetics, which can help enhance input tunability and flexibility in process optimization and control. The light‐responsive transcription factor EL222 from Erythrobacter litoralis is used to regulate protein production from the P C120 promoter in K. phaffii with blue light. We used two system designs to explore the advantages and disadvantages of coupling or decoupling EL222 integration with that of the gene of interest. We investigate the relationship between EL222 gene copy number and light dosage to improve production efficiency for intracellular and secreted proteins. Experiments in lab‐scale bioreactors demonstrate the feasibility of the outlined optogenetic systems as potential alternatives to conventional methanol‐inducible bioprocesses using K. phaffii .