SPR Biosensor Technique Supports Development in Biomaterials Engineering

New Perspectives in Biosensors Technology and Applications 64 plasmon resonance (SPR) technology is a potent analytical tool for biomaterial surface study.This technology makes it possible to prepare a surface of interest (including polymers, metals, ceramics or carbon) and essentially make it the biosensor surface.Subsequently, the kinetics of molecule adsorption to the surface can be observed in real time, without the need for any labeling, together with an extremely high sensitivity of picograms per square millimeter.Moreover, this technique also allows for the identification and quantification of adsorbed molecules by use of specific antibodies.The aim of the present study was to develop conditions that enabled the measurement of plasma protein adsorption to a variety of biomaterials (including Parylene C, nanocrystalline diamond and titanium alloy) using commercially available glass plates pre-coated with gold.The preliminary results obtained regarding plasma protein adsorption were compared with blood platelets adhesion, E. coli and endothelial cells proliferation, as well as changes in proteome of endothelial cells grown on the surfaces of these materials.