荧光
细胞外
肿瘤微环境
材料科学
癌症检测
分子成像
荧光寿命成像显微镜
纳米技术
生物传感器
癌症
癌症研究
化学
生物物理学
生物
细胞生物学
体内
肿瘤细胞
物理
生物技术
量子力学
遗传学
作者
Yiguang Wang,Ke‐Jin Zhou,Gang Huang,Christopher T. Hensley,Xiaonan Huang,Xinpeng Ma,Tian Zhao,Baran D. Sumer,Ralph J. DeBerardinis,Jinming Gao
出处
期刊:Nature Materials
[Springer Nature]
日期:2013-12-08
卷期号:13 (2): 204-212
被引量:697
摘要
Stimuli-responsive nanomaterials are increasingly important in a variety of applications such as biosensing, molecular imaging, drug delivery and tissue engineering. For cancer detection, a paramount challenge still exists in the search for methods that can illuminate tumours universally regardless of their genotypes and phenotypes. Here we capitalized on the acidic, angiogenic tumour microenvironment to achieve the detection of tumour tissues in a wide variety of mouse cancer models. This was accomplished using ultra pH-sensitive fluorescent nanoprobes that have tunable, exponential fluorescence activation on encountering subtle, physiologically relevant pH transitions. These nanoprobes were silent in the circulation, and then strongly activated (>300-fold) in response to the neovasculature or to the low extracellular pH in tumours. Thus, we have established non-toxic, fluorescent nanoreporters that can nonlinearly amplify tumour microenvironmental signals, permitting the identification of tumour tissue independently of histological type or driver mutation, and detection of acute treatment responses much more rapidly than conventional imaging approaches. The imaging of tumours is challenging because of the wide range of different cancers. Now, the rapid detection of tumours, independent of type, is achieved using a nonlinear amplification strategy that employs ultrasensitive pH-responsive fluorescent nanoparticles that illuminate within tumour neovasculature or in response to the tumour’s acidic extracellular environment.
科研通智能强力驱动
Strongly Powered by AbleSci AI