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The Virome of <i>Piper nigrum</i>: Identification, Genomic Characterization, Prevalence, and Transmission of Three New Viruses of Black Pepper in China

胡椒粉 生物 病毒 病毒学 吹笛者 黄瓜花叶病毒 接种 植物病毒 植物
作者
Yuxin Ma,Fei Xing,Haiyan Che,Shengfeng Gao,Yating Lin,Shifang Li
出处
期刊:Plant Disease [Scientific Societies]
卷期号:106 (8): 2082-2089 被引量:1
标识
DOI:10.1094/pdis-12-21-2692-re
摘要

Viral diseases are one of the main categories of diseases that cause substantial yield losses in black pepper. Disease symptoms in black pepper are generally complex and are often caused by both known and undescribed viruses. To identify and clarify the etiology of viral diseases in black pepper in Hainan, China, we conducted high-throughput sequencing (HTS) by targeting purified double-stranded RNA (dsRNA) and ribosomal RNA depleted total RNA (rRNA-depleted totRNA). Analysis of the data revealed the presence of one known virus, piper yellow mottle virus (PYMoV), and three newly identified viruses: black pepper virus F (BPVF) in the genus Fabavirus, black pepper virus E (BPVE) in the genus Enamovirus, and black pepper virus B (BPVB) in the genus Badnavirus. The dominant viruses in P. nigrum sampled in Hainan are PYMoV, with an incidence of 100%, followed by BPVF (84%, 133 of 158) and BPVB (66%, 105 of 158). Mechanical inoculation of sap extracts from source plants containing PYMoV, BPVF, and BPVB gave negative results on both herbaceous and woody host plants 60 days postinoculation (dpi). BPVF and PYMoV were successfully transmitted to virus-free seedlings of black pepper through bark grafting, while BPVB was experimentally undetectable up to 150 dpi. Seed transmission experiments showed that no target viruses were present in all 59 germinated seedlings. This study provides information on diagnosis, prevalence, and transmission of black-pepper-associated viruses.
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