Structures, evolutionary relationships and expression profiles of the tumour necrosis factor superfamily and their receptors in black rockfish (Sebastes schlegelii)

Members of tumour necrosis factor superfamily (TNFSF) and TNF receptor superfamily (TNFRSF) have crucial roles in many important biological processes such as cell proliferation, cell death, development, survival, immunity, and various diseases. The human TNFSF consists of 19 ligands and 29 receptors. Compared with those in human, fish have most of the TNFSF and receptors that have been found in mammals, while some of the homologues are specific or lost in fish. Especially, no systematic report on the identification of TNFSF ligands and their receptors in S. schlegelii. Therefore, to investigate the characterization and molecular evolution of TNFSF and TNFRSF genes in Sebastes schlegelii, we performed a genome-wide survey and identified 14 TNFSFs and 24 TNFRSFs from S. schlegelii. In S. schlegelii, we found duplication events occurred in TNFSF2, TNFSF6, TNFSF10, TNFSF13, TNFSF14, TNFRSF5, TNFRSF6, TNFRSF6B, TNFRSF10B, TNFRSF16, and TNFRSF19 genes. Among which, the tandem duplications events occurred in TNFSF13 and TNFRSF6, and the whole genome duplications events occurred in the remaining TNFSF and TNFRSF genes. Based on the molecular phylogenetic analysis, 14 TNFSFs were divided into three different clusters and 24 TNFRSFs were classed as three distinct subgroups, respectively. Meanwhile, protein domains and motifs analysis revealed that TNFSF contain homology domain (THD), and TNFRSF have typical cysteine-rich domains (CRDs). Synteny results indicates that the TNFSFs and TNFRSFs neighborhood genes have taken place great changes compared to those in human, fugu and zebrafish. Meanwhile, qRT-PCR results demonstrated that most TNFSFs and TNFSRSFs were significantly differentially expressed in gill, skin and intestine after E. tarda infection with time-dependent manners. In addition, protein-protein interaction network (PPI) analysis indicated that the most related genes connecting to TNFSF and TNFRSFs were TNFSF ligands and receptors. In summary, this study provided a new understanding for characterization and evolution of the TNFSF genes and their receptors in S. schlegelii.