Effect of hop mixture containing xanthohumol on sleep enhancement in a mouse model and ROS scavenging effect in oxidative stress-induced HT22 cells

To treat sleep disorders, a compound with sleep-promoting and ROS-scavenging activities is desirable.Hops, which have been used to treat sleep disorders, contain various flavonoids; thus, they may be effective in removing ROS generated during sleep disorders.In this study, the optimal mixing ratios of Simcoe/ Saphir and Saaz/Saphir mixtures for sleep enhancement and ROS-scavenging ability were determined. Materials and methods AnimalsICR male mice (20 ± 2 g; Orient Bio Inc., Seongnam, Korea) were housed at 22 ± 2 °C, relative humidity of 55 ± 5%, ventilation frequency of 10 to 12 h, and a 12 h light-dark cycle.All animal procedures were approved by the Korean University Institutional Animal Care and Use Committee (KUIACUC-2021-0020).