生长素
突变体
MYB公司
生物
细胞生物学
糖基化
植物
光合作用
表型
基因
转录因子
生物化学
作者
Jiacai Chen,Liu Liu,Guangxin Chen,S. P. Wang,Ye Liu,Zeqin Zhang,H. Y. Li,Liming Wang,Zhaoyang Zhou,J. Z. Zhao,Xiaolan Zhang
摘要
ABSTRACT Leaves are the main photosynthesis organ that directly determines crop yield and biomass. Dissecting the regulatory mechanism of leaf development is crucial for food security and ecosystem turn‐over. Here, we identified the novel function of R2R3‐MYB transcription factors CsRAXs in regulating cucumber leaf size and fruiting ability. Csrax5 single mutant exhibited enlarged leaf size and stem diameter, and Csrax1/2/5 triple mutant displayed further enlargement phenotype. Overexpression of CsRAX1 or CsRAX5 gave rise to smaller leaf and thinner stem. The fruiting ability of Csrax1/2/5 plants was significantly enhanced, while that of CsRAX5 overexpression lines was greatly weakened. Similarly, cell number and free auxin level were elevated in mutant plants while decreased in overexpression lines. Biochemical data indicated that CsRAX1/5 directly promoted the expression of auxin glucosyltransferase gene CsUGT74E2 . Therefore, our data suggested that CsRAXs function as repressors for leaf size development by promoting auxin glycosylation to decrease free auxin level and cell division in cucumber. Our findings provide new gene targets for cucumber breeding with increased leaf size and crop yield.
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