小胶质细胞
重编程
代谢途径
生物
糖酵解
细胞生物学
免疫系统
转录组
氧化磷酸化
下调和上调
炎症
TLR4型
信号转导
新陈代谢
生物化学
基因
免疫学
基因表达
作者
Angélica María Sabogal-Guáqueta,Alejandro Marmolejo-Garza,Marina Trombetta-Lima,Asmaa Oun,Jasmijn Hunneman,Tingting Chen,Jari Koistinaho,Šárka Lehtonen,Arjan Kortholt,Justina C. Wolters,Barbara M. Bakker,Bart J. L. Eggen,Erik Boddeke,Amalia M. Dolga
标识
DOI:10.1038/s41467-023-42096-7
摘要
Metabolic reprogramming is a hallmark of the immune cells in response to inflammatory stimuli. This metabolic process involves a switch from oxidative phosphorylation (OXPHOS) to glycolysis or alterations in other metabolic pathways. However, most of the experimental findings have been acquired in murine immune cells, and little is known about the metabolic reprogramming of human microglia. In this study, we investigate the transcriptomic, proteomic, and metabolic profiles of mouse and iPSC-derived human microglia challenged with the TLR4 agonist LPS. We demonstrate that both species display a metabolic shift and an overall increased glycolytic gene signature in response to LPS treatment. The metabolic reprogramming is characterized by the upregulation of hexokinases in mouse microglia and phosphofructokinases in human microglia. This study provides a direct comparison of metabolism between mouse and human microglia, highlighting the species-specific pathways involved in immunometabolism and the importance of considering these differences in translational research.
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