拉曼散射
显微镜
拉曼光谱
调制(音乐)
光学
材料科学
散射
频率调制
光电子学
物理
无线电频率
计算机科学
声学
电信
作者
Fan Wu,Shangyu Li,Xun Chen,Shuhua Yue,Wang Hong,Pu Wang
标识
DOI:10.1002/lpor.202300827
摘要
Abstract Coherent anti‐Stokes Raman scattering (CARS) microscopy has demonstrated a powerful platform for label‐free, non‐invasive, and chemically specific imaging of biological samples. However, the non‐resonant background limits its ability to detect weak Raman signals. Here, an approach to eliminate the non‐resonant background in CARS using delay modulation (DM) is presented, which is enabled by an acousto‐optic modulator and spectral focusing. The results demonstrate that DM‐CARS reduces the non‐resonant background by 10 times and improves detection sensitivity by 100‐fold over normal CARS. It also shows that DM‐CARS has potential applications in tracking heavy water metabolism in bacteria for antimicrobial susceptibility testing and imaging liver tumor tissues. Furthermore, a handheld DM‐CARS device has also demonstrated for in vivo imaging, which can be beneficial in various applications.
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