生物
拟南芥
转录因子
突变体
基因
细胞生物学
遗传学
拟南芥
抑制器
转录调控
调节器
作者
Xiaoyun Li,Guofang Zhang,Liang Yao,Lin Hu,Bonan Zhu,Dongmei Qi,Sujuan Cui,Hongtao Zhao
出处
期刊:Plant Journal
[Wiley]
日期:2021-10-16
卷期号:108 (5): 1493-1506
被引量:22
摘要
SUMMARY The success of plant reproduction depends on the timely transition from the vegetative phase to reproductive growth, a process often referred to as flowering. Although several plant‐specific transcription factors belonging to the Teosinte Branched 1/Cycloidea/Proliferating Cell Factor (TCP) family are reportedly involved in the regulation of flowering in Arabidopsis, the molecular mechanisms, especially for Class I TCP members, are poorly understood. Here, we genetically identified Class I TCP7 as a positive regulator of flowering time. Protein interaction analysis indicated that TCP7 interacted with several Nuclear Factor‐Ys (NF‐Ys), known as the ‘pioneer’ transcription factors; CONSTANS (CO), a main photoperiod regulator of flowering. SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 ( SOC1 ) was differentially expressed in the dominant‐negative mutant of TCP7 ( lcu ) and the loss‐of‐function mutant of Class I TCP members ( septuple ). Additionally, we obtained genetic and molecular evidence showing that TCP7 directly activates the flowering integrator gene, SOC1 . Moreover, TCP7 synergistically activated SOC1 expression upon interacting with CO and NF‐Ys in vivo . Collectively, our results provide compelling evidence that TCP7 synergistically interacts with NF‐Ys to activate the transcriptional expression of the flowering integrator gene SOC1 .
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