中国仓鼠卵巢细胞
铜
细胞毒性
姐妹染色单体交换
有丝分裂指数
金属
中性红
化学
细胞毒性T细胞
仓鼠
细胞培养
中国仓鼠
体外
姐妹染色单体
核化学
分子生物学
有丝分裂
生物化学
生物
细胞生物学
遗传学
受体
染色体
有机化学
基因
作者
Claudia Alejandra Grillo,Miguel A. Reigosa,Mónica Alicia Fernández Lorenzo de Mele
标识
DOI:10.1016/j.mrgentox.2008.09.012
摘要
The aim of this study was to investigate the cytotoxic and genotoxic effect of copper extracts obtained from metallic copper in Chinese hamster ovary (CHO-K1) cell line using neutral red (NR), sister chromatid exchange (SCE), chromosomal aberrations (CA) and cell-cycle kinetics tests. Cells were cultured in Ham-F10 with different copper-containing extracts obtained after the immersion of copper disks for 1, 2, 3, 9, 12, 24, 48 and 72 h in culture medium. Results from cytotoxicity assay showed an inverted U-shape response evidenced in changes in lysosomal activity and mitotic index. The analysis of CA revealed an increase of abnormal metaphases for copper concentration (cCu) in the 5.67-7.42 mg/L dose-range (p<0.001). In addition, SCE frequencies were higher for treated cells when compared with controls in the 1.56-7.42 mg/L concentration range (p<0.001). The absence of metaphases indicated cytotoxicity for cCu≥10.85 mg/L. Results show that cells close to copper-containing materials releasing copper ions are susceptible to cytotoxic and genotoxic effects.
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