Cover Feature: A Systematic Study of Coumarin–Tetrazine Light‐Up Probes for Bioorthogonal Fluorescence Imaging (Chem. Eur. J. 44/2020)

Bioorthogonal fluorogenic reagents that produce after chemical activation fluorescent products represent superior probes for cellular imaging. Due to the improved signal-to-noise ratio, they can be applied in no-wash fluorogenic labeling experiments. The present study reveals a set of coumarin-tetrazine light-up probes, which produce bright dyes in reaction with TCO or BCN dienophile-tagged molecules and enable fast bioorthogonal labeling in live cells. More information can be found in the Full Paper by M. Vrabel et al. on page 9945.