Establishment of animal model of transplanted human colorectal cancer

Objective To establish a stable animal model of transplanted human colorectal cancer. Methods BALB/c nude mice were randomly divided into orthotopic colorectal model group and subcutaneous inoculation model group by random number table, and separately inoculated with 0.1 ml human colon cancer cell HCT116 under the density of 2×107/ml into the orthotopic colorectal (with the self-made inoculator) and right forelimb pit subcutaneously. The mice were observed for 60 days to compare the tumor formation and tumor growth in the two groups. Results The tumor formation rate of all 18 animals was 100% (18/18) in the orthotopic colorectal group. The average tumor weight was (2.78±1.86) g and the average survival time was (45.00±11.99) d. The tumor formation rate was 27.78% (5/18) in the subcutaneous inoculation group. The average tumor weight was (1.74±0.82) g, and the average survival time was (60.00±0.00) d. Conclusion 0.1 ml (2×107/ml) human colon cancer cell suspension HCT116 inoculated into BALB/c nude mice orthotopic colorectal with self-made inoculator could establish human colorectal cancer animal model successfully. Key words: Colorectal neoplasms; Models, animal; Mice, nude; HCT116 cells