蛋白质组学
蛋白质组
基因
转录组
生物
医学
基因表达谱
骨质疏松症
更年期
膜联蛋白A2
基因表达
定量蛋白质组学
破骨细胞
折叠变化
内科学
生物信息学
内分泌学
膜联蛋白
遗传学
细胞
受体
作者
Lan Zhang,Yaozhong Liu,Yong Zeng,Wei Zhu,Yingchun Zhao,Jigang Zhang,Jiaqiang Zhu,Hao He,Hui Shen,Qing Tian,Fei‐Yan Deng,Christopher J. Papasian,Hong‐Wen Deng
出处
期刊:Proteomics
[Wiley]
日期:2015-12-03
卷期号:16 (1): 12-28
被引量:37
标识
DOI:10.1002/pmic.201500005
摘要
Menopause is one of the crucial physiological events during the life of a woman. Transition of menopause status is accompanied by increased risks of various health problems such as osteoporosis. Peripheral blood monocytes can differentiate into osteoclasts and produce cytokines important for osteoclast activity. With quantitative proteomics LC-nano-ESI-MS(E) (where MS(E) is elevated-energy MS), we performed protein expression profiling of peripheral blood monocytes in 42 postmenopausal women with discordant bone mineral density (BMD) levels. Traditional comparative analysis showed proteins encoded by four genes (LOC654188, PPIA, TAGLN2, YWHAB) and three genes (LMNB1, ANXA2P2, ANXA2) were significantly down- and upregulated, respectively, in extremely low- versus high-BMD subjects. To study functionally orchestrating groups of detected proteins in the form of networks, we performed weighted gene coexpression network analysis and gene set enrichment analysis. Weighted gene coexpression network analysis showed that the module including the annexin gene family was most significantly correlated with low BMD, and the lipid-binding related GO terms were enriched in this identified module. Gene set enrichment analysis revealed that two significantly enriched gene sets may be involved in postmenopausal BMD variation by regulating pro-inflammatory cytokines activities. To gain more insights into the proteomics data generated, we performed integrative analyses of the datasets available to us at the genome (DNA level), transcriptome (RNA level), and proteome levels jointly.
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