The proposed work investigates the effects of static magnetic field on blood sample when exposed to non-uniform static magnetic field of 0.35 T. The curves have been plotted between wavelength and transmittance T% with the help of spectrophotometer. Three sets of curves are obtained viz. before placing in magnetic field, after placing in magnetic field of 0.35 T for 20 min and then after 20 min of removing the field. The change in nature of these curves shows that there is an effect of magnetic field on blood. The most probable reasons for these changes are magnetization of blood and increase in energy of blood due to the magnetic field. Thus it can be concluded that the exposure to the magnetic field caused the effect.