骨髓
造血
体内
体外
细胞生物学
干细胞
造血干细胞
祖细胞
生物
免疫学
生物技术
生物化学
作者
Yu-suke Torisawa,Catherine S. Spina,Tadanori Mammoto,Akiko Mammoto,James Weaver,Tracy Tat,James J. Collins,Donald E. Ingber
出处
期刊:Nature Methods
[Springer Nature]
日期:2014-05-04
卷期号:11 (6): 663-669
被引量:363
摘要
Current in vitro hematopoiesis models fail to demonstrate the cellular diversity and complex functions of living bone marrow; hence, most translational studies relevant to the hematologic system are conducted in live animals. Here we describe a method for fabricating 'bone marrow-on-a-chip' that permits culture of living marrow with a functional hematopoietic niche in vitro by first engineering new bone in vivo, removing it whole and perfusing it with culture medium in a microfluidic device. The engineered bone marrow (eBM) retains hematopoietic stem and progenitor cells in normal in vivo-like proportions for at least 1 week in culture. eBM models organ-level marrow toxicity responses and protective effects of radiation countermeasure drugs, whereas conventional bone marrow culture methods do not. This biomimetic microdevice offers a new approach for analysis of drug responses and toxicities in bone marrow as well as for study of hematopoiesis and hematologic diseases in vitro.
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