基因分型
人乳头瘤病毒
基因型
质谱法
细胞学
打字
医学
生物
化学
色谱法
病理
内科学
遗传学
基因
作者
Monica Cricca,Elena Marasco,Federica Alessandrini,Chiara Fazio,Anna Prossomariti,Claudia Savini,Simona Venturoli,Pasquale Chieco,Sabrina De Carolis,Massimiliano Bonafé,Maria Carla Re,Paolo Garagnani,Vilma Mantovani
出处
期刊:PubMed
日期:2015-04-01
卷期号:38 (2): 211-23
被引量:14
摘要
A high-throughput matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)-based method was here developed to genotype 16 high-risk human papillomavirus (HPV) types in cervical cytology specimens. This method was compared to a commercial kit, the Inno-LiPA HPV genotyping assay, which detects a broad spectrum of HPV types. HPV DNA was assessed by the two methods in a total of 325 cervical cytology specimens collected in PreservCyt® solution. The overall agreement was almost perfect (Cohen's k=0.86) in term of positive and negative cases. Indeed, HPV types 16, 35, 56 and 66 showed the highest agreement values (>0.80). The highest agreement values (K >0.80) were found for all 16 HPV types in single infections, but only for HPV 16, 35, 45 and 56 in multiple infections. In conclusion, the high-throughput MS-based method developed here is well-suited for broad spectrum HPV genotyping in large-scale epidemiological studies.
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