Strategy Discussion for CHO Cell Line Development

Several strategies for industrial production of monoclonal antibody drug were introduced in this paper,including host cell selection,construction of expression vector,transfection method,screening techniques,cell culture technology as well as the standard method of final cell lines selection. On the basis of the progress of monoclonal antibody drug CHO cell line development and cell culture process,current strategy for the development of CHO cell lines in monoclonal antibody technology were also discussed.